Device

Part:BBa_K259010:Design

Designed by: Petros Mina   Group: iGEM09_BCCS-Bristol   (2009-10-13)


AraC-RBS-FhuA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 125
    Illegal AgeI site found at 1048
    Illegal AgeI site found at 1186
    Illegal AgeI site found at 1591
    Illegal AgeI site found at 2365
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 107
    Illegal SapI.rc site found at 1090


Design Notes

The part was assembled using standard RFC10 assembly.


Source

The arabinose promoter occurs naturally in E.coli. The RBS is designed from the Anderson family. FhuA also occurs naturally in E.coli.

References